Preparation of Sheath Fluid

Sheath fluid is pumped into the flow chamber to form a laminar flow. Your sample is injected into this stream, cells are separated and aligned by the laminar flow. If it runs out, new sheath has to be prepared by the experimentator.

Materials

Preparation time: 10 minutes

  • 2 L autoclaved Millipore water in a 'frosted glass' bottle (it has to be autoclaved under all circumstances!)
  • 5% Sodium azide solution (highly toxic! handle with care!), FACS room 4 °C
  • Triton-X 100

Preparation

  1. Fill a bit of Millipore water into a 50 mL falcon tube
  2. Drip 10 drops of Triton-X 100 into the tube. Don't shake!
  3. Let it stir on the magnet stirrer until the Triton is completely in solution
  4. Fill this solution into the 2L bottle
  5. Add 4 mL of Sodium azide
  6. Store next to FACS or attach to FACS. Close cap carefully and airtight
Topic revision: r2 - 2011-08-15, TillDettmering
 
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