Standard Turn-OnMeasureTurn-Off Procedure
ALWAYS WEAR BLUE GLOVES WHEN TOUCHING THE INSTRUMENT!
Turning on the device
Sheath should be at least half full
Turn key switch on FACS
Check in logbook if UV lamp is cooling down. If it is cooling down, wait before turning it on!
Start FACS Logbook
Turn on POWER Switch on Laser
Turn key switch on Laser
Only if needed (DNA stain with DAPI or Hoechst): Press button to turn on UV lamp and wait 20 minutes before starting a measurement
Switch the Laser from Standby to Run
Set up sample file OR load earlier measurement
Load settings file
Set Speed to 1
Pull out tube, wait until Status says READY
Put in sample tube until it snaps in
Instrument should start measuring automatically
Ending measurement and shutting down the device
Save your settings file if necessary
Set speed to 20
Fill empty tube at half with green solution and let it run until it stops by itself
Fill empty tube at 3/4 with aq. dest. and let it run until it stops by itself; Leave the tube in the sample holder.
Switch the laser from Run to Standby
Turn the key switch on the laser AND WAIT until the green light is off before turning the POWER switch off
Press STOP button on FACS log book!
Shutdown the computer
This topic: FACS
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