Flow Cytometry at GSI

Information on the status, use, maintenance and data interpretation of the Partec PAS III flow cytometer at GSI.

Introductory information on flow cytometry

• Introductory videos — http://www.invitrogen.com/site/us/en/home/support/Tutorials.html
• The Flow Book — http://flowbook.denovosoftware.com/Flow_Book
• Shapiro's Practical Flow Cytometry book as a free download

Usage of the device

DON'T TOUCH THE DEVICE WITHOUT HAVING HAD A PERSONAL INTRODUCTION!

• Flow Cytometer Logbook - http://strahlenforschung.de/facsbook/index.php

• TechnicalDatasheet
• StandardProcedure for turning on, measuring, and turning off
• StandardSettings to start with
  • QualityControl before every measurement
• Maintenance of the device
  • Proper WasteDisposal
  • Preparation of SheathFluid
• DataAnalysis using FloMax
• CellCycleAnalysis^? using MultiCycle^? AV
• FACSlogBook Help and Version history

Protocols

• DAPIStaining

Troubleshooting

• To be written. Ask Till when in trouble.

Advanced Topics

• CrosstalkCompensation^?